• A Protocol for Single-Molecule Translation Imaging in Xenopus Retinal Ganglion Cells 

      Ströhl, Florian; Lin, Julie Qiaojin; Tartwijk, Francesca W. van; Wong, Hovy Ho Wai; Holt, Christine E.; Kaminski, Clemens F. (Chapter; Bokkapittel, 2020-04-29)
      Single-molecule translation imaging (SMTI) is a straightforward technique for the direct quantification of local protein synthesis. The protein of interest is fused to a fast-folding and fast-bleaching fluorescent protein, allowing one to monitor the appearance of individual fluorescence events after photobleaching of pre-existing proteins in the cell under investigation. The translation of individual ...
    • A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view 

      Opstad, Ida Sundvor; Ströhl, Florian; Fantham, Marcus J.; Hockings, Colin; Vanderpoorten, Oliver; Tartwijk, Francesca W. van; Qiaojin Lin, Julie; Tinguely, Jean-Claude; Dullo, Firehun Tsige; Kaminski‐Schierle, Gabriele S.; Ahluwalia, Balpreet Singh; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-17)
      Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based ...